Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns.
نویسندگان
چکیده
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.
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ورودعنوان ژورنال:
- Physical review letters
دوره 104 22 شماره
صفحات -
تاریخ انتشار 2010